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Arthropods, 2013, 2(4): 208-215
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Article

Selection of entomopathogenic fungi against the red spider mite Tetranychus kanzawai (Kishida) (Tetranychidae: Acarina)

Yayan Sanjaya1 , Virginia R. Ocampo2, Barbara L. Caoili2
1Biology Program, Indonesia University of Education, Jl Setiabudi No. 229, Bandung 40154, Indonesia
2Crop Protection Cluster, College of Agriculture, University Philippines Los Banos, College, Laguna 4031, Philippines

Received 18 July 2013;Accepted 23 August 2013;Published online 1 December 2013
IAEES

Abstract
The pathogenicity of three entomopathogenic fungal species to Tetranychus kanzawai was investigated. Seven isolates of Metarhizium anisopliae, six isolates of Beauveria bassiana, and an isolate of Paecilomyces lilacinus from the Philippines and Indonesia were evaluated. The following studies were undertaken: (1) screening of M. anisoplae, B. bassiana and P. lilicanus pathogenic to T. kanzawai, and (2) bioefficacy studies of the selected entomopathogenic fungi under greenhouse conditions. Conidia of each isolate were mass-produced on potato dextrose agar (PDA) at 26¡À1 oC and a 12-hour photophase for a maximum of 21 days. Preliminary screening for the most pathogenic isolate within the same species was determined using suspension with 104 to 108 conidia ml-1. At 4 days after treatment (DAT), the pathogenicity within M. anisopliae isolates in decreasing order was Ma5>Ma6>Ma4>Ma2>Ma1>Ma3>Ma7 while for B. bassiana, was Bb6>Bb5>Bb4>Bb3>Bb1>Bb2. The top three most pathogenic isolates within the two species were subjected to further studies to determine the most virulent isolate against T. kanzawai. At 5 DAT, the LC50 values of M. anisopliae isolates ranged from 5.0 x102 to 1.4x103 while for B. bassiana ranged from 1.2 x 103 to 2.4x 103 conidia ml-1. Based on LC50, the virulence of the fungal isolates within the species in decreasing order was Ma6>Ma5>Ma4 and Bb6>Bb5>Bb4. However, the LC50 values are not significantly different from each other. Green house trials showed that the epizootic of entomopathogenic fungus can regulate the population of mites. The fungal isolates used in the study, although not originally isolated from mites were virulent to T. kanzawai, indicating their wide host range.

Keywords entomopathogenic fungi;mortality;selection;Tetranychus kanzawai;Beauveria bassiana;Metarhizium anisopliae;greenhouse.


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