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Arthropods, 2016, 5(2): 77-86
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Article

The expression profile of detoxifying enzyme of tomato leaf miner, Tuta absoluta Meyrik (Lepidoptera: Gelechiidae) to chlorpyrifos

Idin Zibaee, Ali Reza Bandani, Ghodratollah Sabahi
Department of Plant Protection, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran

Received 19 February 2016;Accepted 25 March 2016;Published online 1 June 2016
IAEES

Abstract
The tomato leafminer, Tuta absoluta (Meyrich) (Lepidoptera: Gelechiidae) is an important pest of tomato crops worldwide. The persistent use of organophosphate insecticide to control this pest has led to resistance. However, there is no report on the susceptibility and resistance mechanism of field population of Tuta absoluta (Meyrik) from Iran. Furthermore, the toxicity and impact of chlorpyrifos on metabolic enzymes in this pest remains unknown. The populations of T. absoluta from Rasht in Iran displayed LC30; 4332, LC50; 5010 and LC90; 7027 g larva-1 to chlorpyrifos. The toxicity of chlorpyrifos could be synergized more bydiethyl maleate (DEM) and triphenylphosphate (TPP) whereas the synergistic effect of piperonylbutoxide (PBO) was not efficient as well as two other synergists. The synergistic effect ranged from 1.3 to 1.9-fold in 24 h and 1.2 to 1.5-fold in 48 h. The exposure with chlorpyrifos for 24 and 48 h significantly increased the activities of esterase and cytochrome P450-dependent monooxygenases, while there were no significant changes in glutathione-S-transferase. Field populations of T. absoluta from Iran displayed less susceptibility to chlorpyrifos and had a relatively high LC50 in compare to other previous studies. Esterases and cytochrome P450 monooxygenase might be involved in the metabolism, and hence resistance to, chlorpyrifos in this pest.

Keywords tomato leafminer;chlorpyrifos;Tuta absoluta;synergistic effects;resistance.



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