International Academy of Ecology and Environmental Sciences Arthropods 2224-4255 10 1 2021 3 1 Environmental and soil gradient effects on biodiversity of butterflies in Khyber Pakhtunkhwa, Pakistan 1-17 EN Haroon Arif Jan Emmanuel Chidiebere Edeh Mujeeb ur Rahman Zahid Khan Jan Muhammad Hanif Khan Irfan Ullah Ihsan Ali Ihsan Muhammad Tauseef Ahmad LianXi Xing http://www.iaees.org/publications/journals/arthropods/articles/2021-10(1)/environmental-and-soil-gradient-effects-on-biodiversity.pdf> The natural territory disturbance, losses, and degradation are real and alarming threats to the ecosystem. The population of butterflies remains arbitrary in prairie residuals and linear range habitats. Such environments are low, isolated, and sub-quality than intact prairies - biodiversity losses associated with ecosystems regulating services and supporting services. Therefore, the present study was conducted on environmental variables and soil gradient and its effects on butterfly colonies in tehsil Tangi, Charsadda, Khyber Pakhtunkhwa, Pakistan. A total of 506 specimens of butterflies and soil samples were collected from different localities randomly. Butterflies were collected with aerial nets, searching, and picking method, and taken to the laboratory for identification and recording concerning each location every two weeks from August 2014 to May 2015. The similarity index among individuals was 246 (47%), while among species 45.5 (8.62%) and Catopsilia pyranthe were at (35/122; 14/30; 7/14; 6.91/24.11) position, similarly in dissimilarity index, Catopsilia ponoma was at (58/122; 19/30; 9/14; 11.460/24.11) position. The density revealed that the Danauas chrysippus was the most abundant species 122/506. Similarly, among environmental gradients, silt associated considerably with pH, wilting point (Wp), field capacity (Fc), bulk density (Bd), saturated hydraulic conductivity (Shy) and available water (Aw) (p approximately equals to 0.000), sand with pH, Wp, Fc and Bd (p approximately equals to 0.000), pH with Wp, Fc and Bd (p approximately equals to 0.000), Wp with Fc, Bd, and Aw (p approximately equals to 0.000), Fc with Bd and Aw (p approximately equals to 0.000). We concluded that low latitude and altitude, excessive amounts of insecticides and pesticides show a significant impact on butterflies' diversity.