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Arthropods, 2022, 11(4): 176-185
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Article

Extraction and characterization of chitin from granary weevil, Sitophilus granaries L. (Coleoptera: Curculionidae)

Pranil Jagdale1, Nilesh Mharsale2, Rhushikesh Gotarne3, Sujata Magdum1
1PG Department and Research Centre in Zoology, MVP Samaj's KRT Arts, BH Commerce and AM Science (KTHM) College, Nashik, Maharashtra-422002, India
2Department of Physics, MVP Samaj's Arts, Commerce and Science College, Tryambakeshwar, Nashik, Maharashtra-422212, India
3Department of Chemistry, MVP Samaj's Arts, Commerce and Science College, Tryambakeshwar, Nashik, Maharashtra-422212, India

Received 8 July 2022;Accepted 15 August 2022;Published online 1 September 2022;Published 1 December 2022
IAEES

Abstract
Insect chitin has been extracted from granary weevil - Sitophilus granarius L, commonly called wheat weevil in laboratory by treating it with 1 M HCl and 1 M NaOH. This was followed by decolorization. The yield of chitin from this species is 20.2%. The chemical structure and physicochemical properties of -chitin was characterized using Fourier transform infrared (FTIR), X-ray diffraction, scanning electron microscopy. Chitin exhibited similar chemical structures and physicochemical properties to earlier investigated chitin form different insect as well as commercial chitin. The Degree of Acetylation (DA) value for chitin was 97.48% and chitin crystalline index (CrI) was 78.77%. Thus it is a promising alternative for source of chitin.

Keywords Sitophilus granaries;chitin;characterization;FTIR;XRD;SEM.



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