C.C. Cabuga Jr I.C.B. Delabahan J.I.C. Dedel M.A. Ayaton L.A. Ombat M.L.M. Budlayan Graduate School, Caraga State University-Main Campus, Ampayon, Butuan City 8600, Philippines Biology Department, College of Arts and Sciences, Caraga State University- Main Campus-Ampayon, Butuan City 8600 Philippines Physics Department, Mindanao State University-Iligan Institute of Technology, Andres Bonifacio Avenue, Iligan City 9200, Philippines Computational Ecology and Software ISSN 2220-721X http://www.iaees.org/publications/journals/ces/online-version.asp 2018 8 2 46 56 International Academy of Ecology and Environmental Sciences Hong Kong 26 January 2018 5 March 2018 1 June 2018 macrophytes shape analysis aquatic weeds leaf morphology This study was carried out in order to investigate the leaf blade shape variation in the populations of water hyacinth (Eichhornia crassipes) from Lake Mainit, Philippines using geometric morphometric analysis. A total of 120 leaf individuals were randomly collected in the four Barangays (Bunga, San Pablo, Dinarawan and San Roque) of which each area consists of 30 samples. To identify morphological variations in the leaf blade shape of water hyacinth, digital imaging was prepared and the acquired images were loaded into tpsDig2 program. Using thin-plate spline (TPS) series, landmark analysis was completed and subjected to symmetry and asymmetry in geometric data (SAGE) software. Results in Procrustes ANOVA showed high significant differences (P less than 0.0001) in the two factors analyzed: the sides and the individuals by sides which denoting high fluctuating asymmetry. This could be inferred that asymmetric variability might be associated to the outcomes of fluctuating asymmetry that have been derived from genetic and non-genetic influences. Moreover, differences of leaf blade shape have been observed from the collected leaf samples and among the study areas. Thus, using geometric morphometric analysis enables to identify morphological variations within and among species of the same taxa. http://www.iaees.org/publications/journals/ces/articles/2018-8(2)/geometric-morphometrics-of-leaf-blade-shape.pdf