Home

Network Biology, 2013, 3(2): 74-86
[XML] [EndNote Tagged] [BibTex] [ Full PDF (406K)] [Comment Article]

Article

3D structure prediction of replication factor C subunits (RFC) and their interactome in Arabidopsis thaliana

Mohamed Ragab Abdel Gawwad 1, Jasmin Sutkovic1, Emina Zahirovic1, Faruk Berat Akcesme1, Betul Akcesme1, Lizhi Zhang2
1Genetics and Bioengineering department, International University of Sarajevo, Ilidza, 71220 Bosnia and Herzegovina
2Department of Molecular Genetics, The Ohio State University, 484 West 12th Avenue, Columbus, OH 43210, USA

Received 17 January 2013;Accepted 30 January 2013;Published online 1 June 2013
IAEES

Abstract
DNA stress can causes potentially spontaneous genome damage during DNA replication process. Proteins involved in this process are DNA-dependent ATPases, required for replication and repair. In this study the 3-D structure of RFC protein subunits in Arabidopsis thaliana: RFC1, RFC2, RFC3, RFC4 and RFC5 are predicted and confirmed by Ramachadran plot. The amino acid sequences are highly similar to the sequences of the homologous human RFC 140-, 37-, 36-, 40-, and 38 kDa subunits, respectively, and also show amino acid sequence similarity to functionally homologous proteins from E. coli. All five subunits show conserved regions characteristic of ATP/GTP-binding proteins and have significant degree of similarity among each other. The segments of conserved amino acid sequences that define a family of related proteins have been identified. RFC1 is identical to CDC44, a gene identified as a cell division cycle gene encoding a protein involved in DNA metabolism. Subcellular localization and interactions of each protein RFC protein subunit is determined. It subsequently became clear that RFC proteins and their interactome have functions in cell cycle regulation and/or DNA replication and repair processes. In addition, AtRFC subunits are controlling the biosynthesis of salicylic and salicylic acid-mediated defense responses in Arabidopsis.

Keywords DNA;RFC protein;3-D structure;structure prediction;interactome;Arabidopsis thaliana.


International Academy of Ecology and Environmental Sciences. E-mail: office@iaees.org
Copyright © 2009-2024 International Academy of Ecology and Environmental Sciences. All rights reserved.
Web administrator: office@iaees.org, website@iaees.org; Last modified: 2024/5/9


Translate page to: