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Network Biology, 2019, 9(4): 96-106
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Article

The evaluation of class 1 to 3 integrons in Salmonella and antimicrobial resistance pattern isolated from Ross 308 broiler chickens

Mehrnoosh Doosti Irani1, Mostafa Faghani2, Abbas Doosti1
1Biotechnology Research Center, Islamic Azad University, Shahrekord Branch, Shahrekord, Iran
2Department of Animal Science, Faculty of Agriculture, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran

Received 5 September 2019;Accepted 16 September 2019;Published 1 December 2019
IAEES

Abstract
High prevalent of multiple drug resistant (MDR) Salmonella is considered as a threat for human's health. Integrons are one of the most important factors that can contribute to the occurrence of MDR bacteria. The aim of this study was to determine the prevalence of class 1, 2 and 3 integrons among Salmonella strains isolated from broiler chicks. This study was performed on 100 Salmonella isolated strains, collected from male and female broiler chicks samples in southwest of Iran. The prevalence of class 1-3 integrons were verified using specific primers by multiplex PCR assay. Also antimicrobial susceptibility testing by disk diffusion method was performed for each isolate. Screening of Salmonella isolates revealed the prevalence of class 1, 2 and 3 integrons (50%), (28%) and (48%), respectively. Based on the results of this study significant correlations were between MDR and integrons, this is a serious problem in human and veterinary medicine. According to these results Ampicillin was the most resistant antibiotics against Salmonella isolated strains. The resistance to Gentamicin and Tetracycline and Chloramphenicol has increased in the presence of integrons. The presence of all three classes of integrons and its direct connection with the MDR in Salmonella is concerned.

Keywords broiler chicks;integrons;Iran;multiplex PCR;Salmonella.


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