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Network Biology, 2020, 10(2): 32-44
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Article

Interactome analysis and docking site prediction of DNA X-ray repair cross-complementing protein (XRCC) in Arabidopsis thaliana

Mohamed Ragab Abdel Gawwad1, Mohamed Soliman Elshikh2, Haris Lokvancic1
1Department of Genetics and Bioengineering Program, Faculty of Engineering and Natural Sciences, International University of Sarajevo, Sarajevo, Bosnia and Herzegovina
2Department of Botany and Microbiology, College of Sciences, King Saud University, Riyadh 11451, Saudi Arabia

Received 18 January 2020;Accepted 25 February 2020;Published 1 June 2020
IAEES

Abstract
There are seven homologs in eukaryotic RAD51 gene family which are conserved among animals and plants, and those are RAD51, DMC1, RAD51c, XRCC3, RAD51b, RAD51d and XRCC2. The first four of them are important in the process of homologous recombination, but also the DNA repair mechanism, while the other three show normal meiosis. RAD51, DMC1, RAD51c and XRCC3 have lineages that are divergent from the other three paralogs, showing potential functional redundancy. The repair mechanism also includes single- or double-strand break rejoining during replication, recombination and DNA damage, which is made by the DNA ligase enzymes. There are many DNA ligase enzymes, and the sequenced genome of Arabidopsis thaliana showed a homologue XRCC4 of the human DNA ligase IV binding protein. Arabidopsis thaliana encoded also homologues for the other six vertebrate Rad51 proteins. Our Results showed the XRCC2 and XRCC3 are interacting with Rad51c. Tow complexes will be formed; BCDX2 (RAD51B-RAD51C-RAD51D-XRCC2) and CX3 (RAD51C-XRCC3). The two complexes have a function at two distinct stages of homologous recombinational DNA repair.

Keywords DNA damage;DNA repair mechanism;interactome;domain;XRCC protein;RAD51.


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