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Network Biology, 2020, 10(4): 84-91
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Article

Association of VNTR 27-bp polymorphism in intron 4 of the eNOS3 gene and predisposition to Ischemic Heart Disease among Taif population in Saudi Arabia

Adel Qlayel Alkhedaide1, Adil Mergani1,2, Mohamed Mohamed Soliman1,3, Tamer Ahmed Ismail1,4, Samir Ahmed Elshazly5,6, Ayman Sabry7,8
1Medical Laboratory Department, Turabah University College, Taif University, Turabah, P.O. Box 888 Zip Code 21974, Saudi Arabia
2Department of Human Molecular Genetics, National Cancer Institute (NCI), Gezira University, Sudan
3Biochemistry Department, Faculty of Veterinary Medicine, Benha University, Toukh, Egypt
4Physiology Department, Faculty of Veterinary Medicine, Zagazig University, Zagazig, Egypt
5Department of Biotechnology, Faculty of Science, Taif University, Taif, P.O. Box 888 Zip Code 21974, Saudi Arabia
6Biochemistry Department, Faculty of Veterinary Medicine, Kafrelsheikh University, Kafrelsheikh, Egypt
7Department of Biology, Faculty of Science, Taif University, Taif, P.O. Box 888 Zip Code 21974, Saudi Arabia
8National Research Center, El Buhouth St., Zip Code 12622, Dokki, Giza, Egypt

Received 21 June 2020;Accepted 4 September 2020;Published 1 December 2020
IAEES

Abstract
Genetic variation and polymorphism became a hot spot for researches to study the link between societies and certain endemic diseases. This study is an attempt to examine the possible association of the incidence of ischemic heart disease with the genetic variations of 27-bp variable number of tandem repeats located in intron 4 of the eNOS3 gene among Taif population. A case-control study included 81 Ischemic Heart Disease (IHD) patients and 225 unrelated healthy participants from the population living in Taif City. Genotyping of the candidate sequence 27-bp repeat located in intron 4 VNTR of the eNOS3 gene in was conducted using the polymerase chain reaction technology. The minor allele (4a) was slightly less frequent among IHD patients and insignificantly linked to reduced relative risk for IHD. In addition, a significant difference in the distribution of both heterozygous genotype 4a4b between IHD patients and normal groups (p value = 0.012). Presented data suggest that the heterozygous genotype of eNOS3 gene intron 4a4b VNTR variation is might be associated with lowering the risk of IHD in the Taif population in the west of Saudi Arabia. While the minor allele (a) of the eNOS3 gene is insignificantly related to the predisposition of IHD.

Keywords Ischemic Heart Disease;VNTR 27-bp;eNOS;polymorphism;genetic variation.


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