International Academy of Ecology and Environmental Sciences Network Biology 2220-8879 12 4 2022 12 1 Evolutionary aspect of protein sequence network based on the 2D representation of amino acids 133-141 EN Sanjay Sharma Birinchi Kumar Boruah Tazid Ali http://www.iaees.org/publications/journals/nb/articles/2022-12(4)/protein-sequence-network-based-on-2D-representation.pdf> For the comparative analysis of proteins, their proper clustering, and evolutionary relationships require analysis of their sequences. We used a mathematical parameter termed a similar factor to create a similar degree matrix of ND6 protein sequences taken from eight different species in this paper. We built a network out of the matrix to analyze their evolutionary and similarity trends with each other. By observing the various centrality measures, the correlation between multiple centrality measures and different network parameter shows that our network is consistent with the known evolution fact of ND6 protein sequences.