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Ornamental and Medicinal Plants, 2018, 2(1-4): 1-6
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Article

Effect of sucrose supplementation in micro-propagation nodal culture of Sicilian Soapwort (Saponaria sicula)

Sara Sardashti
Department of Agronomy, Faculty of Agriculture, Zabol University, Zabol, Iran

Received 1 August 2018;Accepted 10 September 2018;Published 1 December 2018
IAEES

Abstract
Sicilian Soapwort (Saponaria sicula), is a perennial plant belonging to the family of carnation and a valuable medicinal and industrial plant. The origin of this plant is the Mediterranean region. The medicinal properties include blood filtering, elimination of rheumatism, gout, urinary tract and skin diseases, cure cancer, eczema and tuberculosis. The purpose of this study was to determine the Saponaria sicula micro-propagation using explants inter nodes in a medium containing 50 grams of sucrose in the presence of hormone levels of Benzyl adenine (BA) in range of (0, 0.05, 0.2 and 0.5) and naphthalene acetic acid in range of (0, 0.05 , 0.2 and 0.5). Some factors such as fresh weight, root and stem length, leaf area, length and width of callus, number of stems and roots, embryo genetic plants, aerial root, root hair, green leaves, red leaves, lost leaves, number of nodes and stem produced from each node and color of callus were determined. Results showed that the free-hormone medium conducive to the increment of root length, the medium containing 0.5 mg/l BA and 0.5 mg/l naphthalene acetic acid (NAA) increased the number of red leaves, length of plant, as well as the number of stems growing from each node, the medium without BA, containing 0.5 mg/l NAA increased the number of green leaves, the number of nodes, length and width of leaves, medium containing 0.5 mg/l BA and NAA 0.05 mg per liter gave rise to dead leaves, medium containing 0.5 milligrams per liter BA and without naphthalene acetic acid affected the number of roots, plant fresh weight and length of callus, the medium 0.05 mg/l BA and 0.05 mg/l naphthalene acetic acid increased the length of leaves and the number of embryo genetic plants. The medium containing mg/liter BA with 0.2 mg/l naphthalene acetic acid had an increasing effect on hair roots and the medium containing 0.05 mg/l BA and 0.5 mg per liter NAA were effective in increasing the width of the callus.

Keywords Benzyl adenine;naphthalene acetic acid;Saponaria sicula;sucrose supplementation.

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