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Ornamental and Medicinal Plants, 2022, 5(1-4): 1-11
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Article

Identification of Hemerocallis in vitro culture and estimation of its physiological activities

Eman Tawfik1, Abdulrahman Reda2, Rahma Maher2, Menna-Allah Adel2, Hala Mohamed2, Mohamed Fathy Ahmed3
1Botany and Microbiology Department, Faculty of Science, Helwan University, Egypt
2Biotechnology, Chemistry Department, Faculty of Science, Helwan University, Egypt
3Horticulture Department, Faculty of Agriculture, Ain Shams University, Egypt

Received 2 March 2022;Accepted 15 March 2022;Published 1 June 2022
IAEES

Abstract
Plants, both ornamental and medicinal, have been used for a variety of purposes for millennia. Ornamental plants may benefit the environment, the economy, and people's lifestyles in a variety of ways. Despite the growing popularity of aesthetic plants, some are grown for medical purposes because they contain a variety of bioactive components such as phenolic compounds, carotenoids, antioxidants, essential oils, and other secondary metabolites. A daylily, often known as a day lily, is a flowering plant in the genus Hemerocallis, which belongs to the Asphodelaceae family. Daylilies are perennial plants whose name refers to the short lifespan of their blossoms. The gorgeous flowers and hardiness of many varieties make this species popular all throughout the world.In this study, the plant species was identified by molecular tools (18s rDNA), the total physiological parameters were measured, the fractions were identified using liquid chromatography - mass spectroscopy LC-MS, and the antimicrobial activity were evaluated resulting in 7 fractions. All the defined compounds were proved to have many activities Hemerocallis. These compounds were used for treatment of many diseases and in traditions applications.

Keywords Hemerocallis;tissue culture;LC-MS;phenolics;flavonoids;chlorophyll pigmentation;18S rDNA;antimicrobial assay.



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