Home

Proceedings of the International Academy of Ecology and Environmental Sciences, 2013, 3(2): 101-132
[XML] [EndNote Tagged] [BibTex] [ Full PDF (172K)] [Comment Article]

Article

Toxicity analysis of pesticides on cyanobacterial species by 16S rDNA molecular characterization

J. I. Nirmal Kumar1, Anubhuti Bora1, Rita N. Kumar2, Manmeet Kaur Amb1, Shamiyan Khan1
1P.G. Department of EnvironmentaScience and Technology, Institute of Science and Technology for Advanced Studies and Research (ISTAR), Vallabh Vidyanagar -388 120, Gujarat, India
2Department of Biosciences and Environmental Science, Natubhai V Patel College of Pure and Applied Sciences (NVPAS), Vallabh Vidyanagar -388 120, Gujarat, India

Received 18 March 2013;Accepted 22 April 2013;Published online 1 June 2013
IAEES

Abstract
Damaging effects of endosulfan on native structure of DNA, evident as a result of PCR based assay such as 16S rDNA amplification and sequencing, led to formation of gaps, mismatching of base pairs and dissimilarities in entire 16S rDNA sequences of treated cultures. Endosulfan was the most fatal to Westiellopsis prolifica of 16S rDNA region at 40ppm insecticide induced series of mispairing, and other lesions amounting up to 20% dissimilarity and 7% gaps. Whereas, 16S rDNA region of Anabaena fertilissima was comparatively less influenced with 18% dissimilarity and 7% gaps in response to 12ppm endosulfan, while 16S rDNA gene of Aulosira fertilissima was the least prone to changes with 17% dissimilarity, and 5% gaps under 60ppm endosulfan stress by the end of 16 days. On the other side, impact of fungicide tebuconazole after 16 days reflected identities up to 78% and 8% gaps for 30ppm treated A. fertilissima, while 60ppm treatment instilled 79% similarities with 10% gaps in W. prolifica and 83% identities with 5% gaps of Aulosira fertilissima after 16 days.

Keywords cyanobacteria species;endosulfan;tebuconazole;16s rDNA molecular characterization.


International Academy of Ecology and Environmental Sciences. E-mail: office@iaees.org
Copyright © 2009-2024 International Academy of Ecology and Environmental Sciences. All rights reserved.
Web administrator: office@iaees.org, website@iaees.org; Last modified: 2024/4/26


Translate page to: