Home

Proceedings of the International Academy of Ecology and Environmental Sciences, 2017, 7(1): 12-19
[XML] [EndNote] [RefManager] [BibTex] [ Full PDF (209K)] [Comment/Review Article]

Article

Allium cepa test: An evaluation of genotoxicity

Cresencio C. Cabuga Jr.1,2, Julene Joy Z. Abelada1,2, Rene Rose Q. Apostado1,2, Brent Joy H. Hernando2, John Erick C. Lador2, Owen Lloyd P. Obenza2, Christian James R. Presilda2, Honelyn C. Havana3
1Graduate School, Caraga State University-Ampayon, Butuan City, 8600 Philippines
2Biology Department, College of Arts and Sciences-Caraga State University-Main Campus, Ampayon, Butuan City, 8600 Philippines
3Cagbas Elementary School, Bayugan City, 8502 Philippines

Received 14 October 2016;Accepted 20 November 2016;Published 1 March 2017
IAEES

Abstract
The importance of Allium cepa test contributes knowledge in preventing toxicity in the environment. This test widely used in determining genotoxic and cytotoxic substances found in the water system. In this study, the genotoxic effects of H2O2 (Hydrogen Peroxide) and CH2O (Formalin) were determined using Allium cepa root tip cells while the water samples collected from the mining areas of Sorex Barobo, Surigao del Sur and Rosario, Agusan del Sur were also tested for genotoxicity. One-Way ANOVA shows that water samples treated with H2O2 were statistically significant (P<0.05) when compared to CH2O while the water sample collected from the mining areas shows statistically non-significant. A concentration-dependent increased were observed among the dividing cells and aberrant cells of the treated and collected water samples. The observed abnormalities were seen into its roots morphology indicating genotoxicity. Thus, the obtained results in this study show that Allium cepa test is useful as bio-indicator to detect genotoxicity.

Keywords Allium cepa;genotoxicity;chromosomal aberration;Agusan del Sur;Surigao del Sur.


International Academy of Ecology and Environmental Sciences. E-mail: office@iaees.org
Copyright © 2009-2024 International Academy of Ecology and Environmental Sciences. All rights reserved.
Web administrator: office@iaees.org, website@iaees.org; Last modified: 2024/4/27


Translate page to: