International Academy of Ecology and Environmental Sciences Proceedings of the International Academy of Ecology and Environmental Sciences 2220-8860 15 4 2025 12 1 Colchicine-induced morphological and cytological changes in potato (Solanum tuberosum L.) root tips 132-140 EN Marvin James R. Coloma Janiah Faith M. Guerrero Aliyah Lhuk B. Piawan Jerick A. Viz http://www.iaees.org/publications/journals/piaees/articles/2025-15(4)/colchicine-induced-morphological-and-cytological-changes.pdf> Artificial polyploidization through colchicine treatment can enhance plant morphological traits. This study evaluated the effects of six colchicine concentrations (0, 0.5, 1, 2, 4, and 8 mg/mL) on root length, root base diameter, root surface area, cell size, nucleus size, and chromosome number in locally grown potato (Solanum tuberosum L.) collected from Laoag City, Ilocos Norte, Philippines. Root samples were analyzed through quantitative measurements and microscopic examination after fixation and staining, with statistical differences determined using one-way ANOVA. The 1 mg/mL treatment (T2) consistently produced the highest values for all measured parameters and showed significant differences (p less than 0.05) compared to other treatments and the control. Observed increases in cell and nucleus size suggest possible polyploidy induction, although chromosome counts could not be confirmed due to staining limitations. Overall, optimal colchicine concentrations improved both macroscopic and microscopic traits, whereas higher doses were detrimental. These results identify 1 mg/mL colchicine as a promising concentration for enhancing root traits in potato, warranting further whole-plant evaluation for varietal improvement.